Purification of an acid α-glucosidase by dextran-gel filtration
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Purification of an acid alpha-glucosidase by dextran-gel filtration.
1. The behaviour of rat liver alpha-glucosidases on dextran gel (Sephadex G-100) columns was studied. A ;retardation' of an acid alpha-glucosidase activity was observed. This activity was identified as lysosome alpha-(1-->4)-glucosidase. A single gel-filtration step resulted in a 700-fold purification of the enzyme. The same technique was also used to purify the acid alpha-glucosidase of human ...
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Amer. J. Med. 27,963 (1959). [7] GORDON, R.: Exsudative enteropathy. Lancet 191)9/1, 325. [8] SCHWARTZ, M., and B. TROMSEN: Idiopathic or hypcrcatabolic hypoproteinaemia. Brit. med. J. 191)7 I, 14. [9] BARANDUN, S., R. AEBERsoLD u. R. BIANCRI: "Proteindiarrhoe". Schweiz. med. Wschr. 90, 1458 (1960). [10] HANIOKI,Z., J.HAWIGER, P.HIRSZEL, and Z. TWARDOWSKI: On some aspects of the antibody defici...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1967
ISSN: 0306-3283
DOI: 10.1042/bj1050035